Stratagem of DNA Chip Preparation
نویسندگان
چکیده
منابع مشابه
Comparison of sample preparation methods for ChIP-chip assays.
A single chromatin immunoprecipitation (ChIP) sample does not provide enough DNA for hybridization to a genomic tiling array. A commonly used technique for amplifying the DNA obtained from ChIP assays is ligation-mediated PCR (LM-PCR). However; using this amplification method, we could not identify Oct4 binding sites on genomic tiling arrays representing 1% of the human genome (ENCODE arrays). ...
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ژورنال
عنوان ژورنال: Kobunshi
سال: 2004
ISSN: 0454-1138,2185-9825
DOI: 10.1295/kobunshi.53.880